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Inquiries that began with the Geissler tube continued as even better vacuums were produced. The most famous was the evacuated tube used for scientific research by William Crookes. That tube was evacuated by the highly effective mercury vacuum pump created by Hermann Sprengel. Research conducted by Crookes and others ultimately led to the discovery of the electron in 1897 by J. J. Thomson. But the Crookes tube, as it came to be known, produced little light because the vacuum in it was too good and thus lacked the trace amounts of gas that are needed for electrically stimulated luminescence.

    The next step in gas-based lighting took advantage of the luminescent qualities of neon, an inert gas that had been discovered in 1898 by isolation from the atmosphere. Neon glowed a brilliant red when used in Geissler tubes. By 1910, Georges Claude, a Frenchman who had developed a technology and a successful business for air liquefaction, was obtaining enough neon as a byproduct to support a neon lighting industry.

    In 1934, Arthur Compton, a renowned physicist and GE consultant, reported to the GE lamp department on successful experiments with fluorescent lighting at General Electric Co., Ltd. in Great Britain (unrelated to General Electric in the United States). Stimulated by this report, and with all of the key elements available, a team led by George E. Inman built a prototype fluorescent lamp in 1934 at General Electric's Nela Park (Ohio) engineering laboratory.

    After years of work, Moore was able to extend the operating life of the lamps by inventing an electromagnetically controlled valve that maintained a constant gas pressure within the tube.Although Moore's lamp was complicated, expensive to install, and required very high voltages, it was considerably more efficient than incandescent lamps, and it produced a more natural light led tube than incandescent lamps. From 1904 onwards Moore's lighting system was installed in a number of stores and offices. Its success contributed to General Electric's motivation to improve the incandescent lamp, especially its filament.

While the Hull patent gave GE a basis for claiming legal rights over the fluorescent lamp, a few months after the lamp went into production the firm learned of a U.S. patent application that had been filed in 1927 for the aforementioned "metal vapor lamp" invented in Germany by Meyer, Spanner, and Germer. The patent application indicated that the lamp had been created as a superior means of producing ultraviolet light, but the application also contained a few statements referring to fluorescent illumination.

The light output and performance of fluorescent lamps is critically affected by the temperature of the bulb wall and its effect on the partial pressure of mercury vapor within the lamp. Each lamp contains a small amount of mercury, which must vaporize to support the lamp current and generate light. At low temperatures the mercury is in the form of dispersed liquid droplets.

    Fluorescent lamp tubes are typically straight and range in length from about 100 millimeters (3.9 in) for miniature lamps, to 2.43 meters (8.0 ft) for high-output lamps. Some lamps have the tube bent into a circle, used for table lamps or other places where a more compact light source is desired. Larger U-shaped lamps are used to provide the same amount of light in a more compact area, and are used for special architectural purposes.